Immunohistochemical Evaluation Of Cd4, Cd8 And Matrix Metalloproteinase-9 (Mmp-9) Expressions In Biopsies Before And After Treatment Of Mycosis Fungoides Patients

Several studies reported increased CD4/CD8 ratio in patients with mycosis fungoides (MF). A parallel relationship was indicated between tumor progression and elevated matrix metalloproteinase (MMP) levels. The aim of this study was to evaluate levels of CD4, CD8 and MMP-9, which is an indicator of tumoral invasion, in before and after treatment of MF patients. A total of 10 control patients and 32 patients with plaque stage MF pathologically diagnosed as nonspecific chronic dermatosis were included. Biopsies were taken at the Department of Dermatology, Meram Faculty of Medicine, Selçuk University. Phototherapy was performed for 3 months on all patients diagnosed with MF and control biopsies were taken at the end of 3 months. In 20 patients, there were no findings in favor of MF was diagnosed. MF pathological findings were observed in the other 12 patients. For histopathological examination, sections were taken from a sufficient number of control groups and as well as groups of MF patients before and after treatment. CD4, CD8 and MMP-9 immunohistochemical staining was performed at the Department of Pathology, Meram Faculty of Medicine. Intraepithelial MMP-9 and subepithelial CD4/CD8 values were found to be significantly higher in MFA patients having no benefit from the treatment. It was determined that subepithelial MMP-9, intraepithelial and subepithelial CD4/CD8 values were significantly lower after treatment in the MF patients who had benefit for the treatment. Comparing with the controls, CD4/CD8 and MMP 9 levels were significantly higher in MF patients, and these levels were observed to be decreased in patients who respond well to treatment. Findings of this study were concordant with literature.